Mitochondrial D-loop sequences and haplotypes diversity in Egyptian rabbit breeds

Rabbit breeds in Egypt are local and adapted foreign that have been imported since the middle of last century. Stressful environmental conditions including climatic changes, exposure to diseases breeding selection an influence on how gene flow has shaped genetic diversity breeds. Mitochondrial DNA D-loop is a marker used trace geographic distribution variation for investigation expansions, migrations other patterns. The study aimed determine mitochondrial (mtDNA D-loop) Black Baladi, Red Gabali, APRI line New Zealand gather scientific data required create proper conservation sustainable management plan. Blood samples were taken from animals unrelated each other. A 332-bp mtDNA was successfully amplified alignment sequences deposited GenBank database. results detected six haplotypes five Haplotype within individual varied 0 (Red Baladi) 0.551±0.114 (Gabali). nucleotide (π) value relatively low (0.001-0.006), with greater values Zealand. Pairwise distances between yielded varying ranging 0.254, Baladi comparatively high, pairwise 0.172 0.254. phylogenetic analysis involved 74 Egyptian rabbit thirty-one retrieved reference different haplogroups. correlated database showed grouped into A, B K. using shed light importance breed’s information revealed unique haplotypes, which important finding strategies designed conserve variants provide management.